Restriction digests of the clone give the following sizes (kb): BamHI--3.7, 0.5; BglI--2.7, 1.4; EcoRI--3.6, 0.6; PstI--3.1, 1.1; PstI/BamHI--3.1, 0.6, 0.5; BamHI/BglI--2.3, 1.4, 0.4. The insert includes the following restriction sites in kb from the 5' end (nt position according to the published sequence): BamHI--0.42 (886), EcoRI--0.5 (970). Detects a StuI fragment of 1.5 kb in genomic BALB/cJ DNA and mRNA of 2.0 kb in mouse embryos and adult tissues. A PstI fragment extending from nt 470 to 1514 of the published sequence. It includes coding sequences for the zinc finger domain. |
