| 產品名稱 |
mp40 |
| 商品貨號 |
B191401 |
| Designations |
mp40 |
| GenBank Number |
M86671
|
| Species |
Mus musculus, mouse |
| Depositors |
U Gubler |
| Vector |
Construct size (kb): 4.013999938964844 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript SK+
Intact vector size: 2.964
Type of vector: phagemid
Vector end: HindIII
Vector end: EcoRI
Cloning sites: KpnI Eco0109I ApaI DraII XhoI SalI HincII AccI ClaI Bsp106I
HindIII EcoRV EcoRI PstI SmaI BamHI SpeI XbaI EagI NotI
SacII BstXI SacI
Polylinker sites: KpnI Eco0109I ApaI DraII XhoI SalI HincII AccI ClaI Bsp106I
HindIII EcoRV EcoRI PstI SmaI BamHI SpeI XbaI EagI NotI
SacII BstXI SacI
Construction: pUC19
Host range: Escherichia coli
Features (with orientation and position when available):
enhancer: none
insert detection: lacZ'
marker(s): ampR
promoter: lac, T3, T7
replicon: pMB1, f1
terminator: none
Cross references: |
| Insert |
DNA: cDNA DESCRIPTION OF INSERT COMPONENT:
Genome: mouse
Gene symbol: Il12b
Genomic copy number: unique
Gene name: interleukin 12B (natural killer cell stimulatory factor 2,
cytotoxic lymphocyte maturation factor 2, p40)
Contains complete coding sequence?: Y
Chromosome: 11
Strain: C57BL/6
Tissue: spleen
Type of DNA: cDNA
Insert 5' end: HindIII
Insert 3' end: EcoRI
Insert size (kb): 1.05
Cross references: DNA Seq. Acc.: M86671 Insert lengths(kb): 1.049999952316284 Tissue: spleen Gene product: interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2, p40) [Il12b] |
| Insert Size (kb) |
1.050 |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information |
Distributed: freeze-dried |
| Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI/HindIII--3.2, 1.1; BamHI--4.3; XbaI--3.2, 0.84, 0.3. The insert contains the following restriction sites (approximate kb from the 5' end): PstI--0.55, 1.02; AvaI--0.52; PvuII--0.79. The sequence surrounding the initiation codon was modified to 5'-ACCAUGG-3' (Kozak consensus) to optimize translation. Thus the sequence between the vector insertion site and the initiating ATG is not derived from the mRNA. |
| Classification |
Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, Tetrapod |
| References |
Schoenhaut DS, et al. Cloning and expression of murine IL-12. J. Immunol. 148: 3433-3440, 1992. PubMed: 1350290
|
