| 產品名稱 |
clone 2.72F, #19 |
| 商品貨號 |
B191793 |
| Designations |
clone 2.72F, #19 |
| GenBank Number |
M28249
|
| Species |
Homo sapiens, human |
| Depositors |
Q Yuan, ME Hemler |
| Vector |
Construct size (kb): 8.329999923706055 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript KS+
Intact vector size: 2.964
Type of vector: phagemid
Vector end: EcoRI closer to XhoI site
Vector end: EcoRI
Cloning sites: SacII XmaII NotI XbaI SpeI BamHI SmaI PstI EcoRI EcoRV
HindIII ClaI (SalI HincII AccI) XhoI DraII ApaI KpnI
Polylinker sites: SEE COMMENTS
Construction: pUC19
Host range: Escherichia coli
Features (with orientation and position when available):
enhancer: none
insert detection: lacZ'
marker(s): ampR
promoter: lac, T3, T7
replicon: pMB1, f1
terminator: none
Cross references: |
| Insert |
DNA: cDNA DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: ITGA2
Genomic copy number: unique
Gene name: integrin, alpha 2 (CD49B, alpha 2 subunit of VLA-2
receptor)
Contains complete coding sequence?: Y
Chromosome: 5; Localization: 5 q23-q31
Tissue: lung fibroblast IMR-90 cell line
Type of DNA: cDNA
Insert end: Modification: EcoRI linker
Insert end: Modification: EcoRI linker
Insert size (kb): 5.367
Cross references: DNA Seq. Acc.: M28249
Nucleotides 1-5367 of the insert correspond to
nucleotides 2-5368 of M28249. Insert lengths(kb): 5.367000102996826 Tissue: lung fibroblast IMR-90 cell line Gene product: integrin, alpha 2 (CD49B, alpha 2 subunit of VLA-2 receptor)(collagen receptor, platelet gpIa/IIa) [ITGA2] |
| Insert Size (kb) |
5.367 |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information |
Distributed: freeze-dried |
| Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI--3.6, 3.0, 1.8; HindIII--3.2, 3.2, 2.1; XbaI--8.4; XhoI--4.8, 3.5; KpnI--4.8, 3.5. The insert contains the following restriction sites (approximate kb from the 5' end): EcoRI--1.79, BamHI--1.86; HindIII--2.02, 5.2. The coding region is nt 49-3594. |
| References |
Takada Y, Hemler ME. The primary structure of the VLA-2/collagen receptor alpha 2 subunit (platelet GPIa): homology to other integrins and the presence of a possible collagen-binding domain. J. Cell Biol. 109: 397-407, 1989. PubMed: 2545729
|
