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Size (kb): 8.3999996185302730

Vector: YEp367R (plasmid)

Promoters: Promoter none

Construction: YEp363A, YEp357R (ATCC 37738)

Marker(s):LEU2,ampR

Construct size (kb): 8.399999618530273

Features: insert detection: lacZ
marker(s): ampR, LEU2
promoter: none
replicon: pMB1, 2 micron

YE-type (episomal) shuttle vector

promoter-cloning vector

shuttle vector

The cleavage position in the reading frame for cloning sites is (where 3 = between triplets): HindIII-3; SphI-1; PstI-1; SalI-3; XbaI-3; BamHI-3; SmaI-1; KpnI-1; SacI-1; EcoRI-3. The EcoRI, KpnI, and SacI sites are not unique.

Restriction digests of the clone give the following sizes (kb): HindIII-8.4; BamHI-8.4; PstI-8.4.

One of 3 promoter-cloning, YE type shuttle vectors (ATCC 37740 - 37742) with LEU2 selection in Saccharomyces cerevisiae, a beta-galactosidase reporter gene and multiple cloning sites differing in reading frame.

The sequence and reading frame of the multiple cloning sequence is: 5'A AGC TTG CAT GCC TGC AGG TCG ACT CTA GAG GAT CCC CGG GTA CCG AGC TCG AAT TCC CAG CTT GCT CCC3', from nucleotide 1 of the MCS through CCC for amino acid 8 of beta-galactosidase.

Temperature: 37.0°C