| Comments |
Restriction digests of the clone give the following sizes (kb): SacI--3.0, 0.6, 0.55; PvuII--2.5, 0.7, 0.45, 0.25; SalI--3.5, 0.8; PstI--3.3, 0.9; XbaI/BglI--4.1; EcoRI/XbaI--2.7, 1.3. The insert contains the following restriction sites [nt from the 5' end (sequence record)]: SacI--211 (1856), 787 (2432); PvuII--249 (1894), 526 (2230), 1273 (2918); NcoI--426 (2171); SalI--787 (2432); PstI--808 (2453). Contains the complete coding sequence (nt 2173-2580) plus 5' and 3' flanking regions. Corresponds to nt 1645-2956 of sequence accession number X16148. |
| References |
Taylor JD, et al. Sequences of four mouse histone H3 genes: implications for evolution of mouse histone genes. J. Mol. Evol. 23: 242-249, 1986. PubMed: 3027355
William F Marzluff, personal communication
|
