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Size (kb): 6.6599998474121090

Vector: pBT142 (plasmid)

Promoters: Promoter none

Construction: pUB110, pNM481

Marker(s):bleR,kanR,neoR

Construct size (kb): 6.659999847412109

Features: insert detection: lacZ'
marker(s): kanR, neoR, bleR
promoter: none
replicon: pUB110

shuttle vector

vector permitting construction of fusion proteins

Restriction digests of the clone give the following sizes (kb): EcoRI--6.6; SmaI--6.6; BamHI--6.6; SalI--6.6; PstI--6.6.

bleR = bleomycin-pheomycin resistance

One of a series (ATCC 37637 - 37639) of 3 plasmid vectors for characterization of regulatory systems in Bacillus subtilis. Inserted genes can be fused to Escherichia coli lacZ, with one vector in the series for each of the 3 translational reading frames.

A 3.1 kb EcoRI/DraI fragment of pNM481 was ligated to a 3.5 kb EcoRI/PvuII fragment of pUB110.

Temperature: 30.0°C