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Not all bands in our restriction analysis show equal intensity.

Construct size (kb): 5.320000171661377

DNA: cDNA

Insert lengths(kb): 0.9369999766349792

Tissue: HuT-102B2 cell line

Gene product: interleukin 2 receptor, alpha( IL-2R alpha chain, Interleukin-2 receptor, IL-2 receptor) [IL2R]

Restriction digests of the clone give the following sizes (kb): EcoRI--4.4, 0.92; HindIII--5.2, 4.4; PstI--4.4, 3.9, 1.2; AvaI--4.4, 5.2; BamHI--4.4, 5.2.

The plasmid contains multiple copies of the pBR322 sequence. Not all bands in our restriction analysis show equal intensity. The following bands are fainter: HindIII--5.2, PstI--3.9, 1.2; AvaI--5.2; BamHI--5.2.

This corresponds to bases 1-937 of pIL2R3. It has artificial EcoRI linkers at the 3' and 5' ends.