| 產品名稱 |
pAS2 [pAS1-CYH2] |
| 商品貨號 |
B192547 |
| Designations |
pAS2 [pAS1-CYH2] |
| Depositors |
SJ Elledge |
| Biosafety Level |
1 |
| Vector Information |
Size (kb): 8.5000000000000000 Vector: pAS2 (phagemid) Promoters: Promoter ADC1 (ADH) Construction: pAS1 Marker(s):TRP1,ampR,cyhS Features: marker(s): CYH2
marker(s): TRP1
marker(s): ampR
other: GAL4 DNA-binding domain
promoter: ADC1 (ADH)
replicon: 2 micron
replicon: f1+
replicon: pMB1
MCS: NdeI...SalI
terminator: ADC1
epitope tag: hemagglutinin (HA) |
| Applications |
YE-type (episomal) shuttle vector contains sequence ribosomal protein L29 Ribosomal protein RL43 expression vector shuttle vector vector for constructing cDNA libraries vector for other uses |
| Comments |
Restriction digests of the clone give the following sizes (kb): BamHI--8.6; SalI--8.6; SmaI--8.6. Shuttle expression vector used to create fusion proteins consisting of the nuclear localization sequence from SV40 T antigen, the GAL4 DNA-binding domain (aa 1-147), and a HA (hemagglutinin) epitope tag in frame with the activation domain. |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Growth Conditions |
Temperature: 37.0°C |
| References |
Harper JW, et al. The p21 Cdk-interacting protein Cip1 is a potent inhibitor of G1 cyclin-dependent kinases. Cell 75: 805-816, 1993. PubMed: 8242751
|
| Shipped |
freeze-dried |
| Shipping Information |
?Freeze dried E. coli containing the plasmid |
