| Comments |
The Melanocyte Growth Kit contains components that when added to Dermal Cell Basal Medium (ATCC PCS-200-030) create a complete low-serum culture environment for melanocytes derived from normal human epidermis (e.g., Primary Epidermal Melanocytes, Normal, Human Neonatal, ATCC PCS-200-012). The complete low serum medium (less than 1.0% FBS) is formulated to support robust melanocyte growth in the absence of cholera toxin and phorbol 12-myristate 13-acetate (PMA). No feeder layers, extracellular matrix proteins or other substrares are required. The final concentration for each component in complete melainocyte growth medium is as follows:
- rh Insulin: 5 µg/ml
- Ascorbic Acid: 50 µg/ml
- L-Glutamine: 6 mM
- Epinephrine: 1.0 µM
- Calcium chloride: 0.2 mM
- M8 Supplement: Proprietary formulation
|
