Restriction digests of the clone give the following sizes (kb): HindIII--2.95, 2.35; EcoRI--2.8 doublet. The insert corresponds to the 3' flanking region of the gene. The probe hybridizes to two HindIII-digested human DNA fragments, a strong 2.3 kb band and a weak 4.0 kb band. Recorded as not polymorphic by Collaborative Research, meaning either not polymorphic in 0.8% agarose gels, or difficult to work with. The insert is an EcoRI fragment derived from lambda14A. The 3' EcoRI site is of phage origin. Enzyme(s) not detecting polymorphism: EcoRI, BamHI, HindIII, XbaI, TaqI, MspI, RsaI, PvuII, BglI, BglII, SmaI. |
