| 產品名稱 |
PHL |
| 商品貨號 |
B193402 |
| Organism |
Clupea pallasi, Pacific herring |
| Tissue |
larvae |
| Cell Type |
epithelial |
| Product Format |
frozen |
| Morphology |
epithelial |
| Culture Properties |
adherent |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Age |
newly hatched larvae larva |
| Applications |
In addition, it can be used to study the VHS virus. This cell line is useful for investigation of the toxicity of naphthalene and other petroleum components to juvenile herring. The PHL (Pacific Herring Larva) cell line was established from newly hatched larvae by explant culture. PHL cells are susceptible to infection by the North American strain of viral hemorrhagic septicemia (VHS) virus, and are sensitive to the cytotoxic effects of naphthalene, a common environmental contaminant. |
| Storage Conditions |
liquid nitrogen vapor phase |
| Karyotype |
The cells retain a diploid karyotype beyond 65 population doublings. |
| Derivation |
Waterloo Ontario, Canada |
| Virus Susceptibility |
Viral hemorrhagic septicemia virus
|
| Comments |
The PHL (Pacific Herring Larva) cell line was established from newly hatched larvae by explant culture.
These cells retain a diploid karotype after 65 population doublings. PHL cells are susceptible to infection by the North American strain of viral hemorrhagic septicemia (VHS) virus, and are sensitive to the cytotoxic effects of naphthalene, a common environmental contaminant. This cell line is useful for investigation of the toxicity of naphthalene and other petroleum components to juvenile herring. In addition, it can be used to study the VHS virus. |
| Complete Growth Medium |
Leibovitz's L-15 medium with 2 mM L-glutamine, 85%; fetal bovine serum, 15% (Note: The L-15 medium formulation was devised for use in a free gas exchange with atmospheric air. A CO2 and air mixture is detrimental to cells when using this medium for cultivation)
|
| Subculturing |
Protocol: - Remove and discard culture medium.
-
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
-
Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach.
Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. -
Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
-
Add appropriate aliquots of the cell suspension to new culture vessels.
- Incubate cultures at 21°C.
Interval: Maintain cultures at a cell concentration between 4 X 10(3) and 5 X 10(4) cells/cm2. Subcultivation Ratio: A subcultivation ratio of 1:3 is recommended Medium Renewal: Every 2 to 3 days |
| Cryopreservation |
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions |
Atmosphere: air, 100% Temperature: 21.0°C; (Max. 21.0 °C, Min. 18.0°C) |
| Population Doubling Time |
about 34 hrs |
| Name of Depositor |
NC Bols |
| Deposited As |
Clupea pallasi |
| References |
Ganassin RC, et al. Development and characterization of a cell line from Pacific herring, Clupea harengus pallasi, sensitive to both naphthalene cytotoxicity and infection by viral hemorrhagic septicemia virus. Cell Biol. Toxicol. 15: 299-309, 1999. PubMed: 10813363
The PHL (Pacific Hering Larva) cell line was established from newly hatched larvae by explant culture. The cells are susceptible to infection by the North American strain of Viral hemorrhagic septicemia (VHS) virus, and are sensitive to the cytotoxic effects of napthalene (a common environmental contaminant).
|
