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Size (kb): 5.3439998626708980

DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pJK142
Intact vector size: 3.272
Type of vector: plasmid
Vector end: NdeI
Vector end: NdeI
Cloning sites: SacI SacII SpeI XbaI NotI BamHI SmaI PstI EcoRI EcoRV
HindIII ClaI SalI HincII XhoI ApaI DraII KpnI
Polylinker sites: SacI SacII SpeI XbaI NotI BamHI SmaI PstI EcoRI EcoRV
HindIII ClaI SalI HincII XhoI ApaI DraII KpnI
Host range: Escherichia coli
Features (with orientation and position when available):
insert detection: lac
marker(s): ampR
promoter: lac
replicon: pMB1
Cross references:

Vector: pJK148 (plasmid)

Promoters: Promoter lac

Construction: pJK142

Marker(s):ampR,leu1

Construct size (kb): 5.343999862670898

Features: insert detection: lacZ'
marker(s): ampR
marker(s): leu1+
promoter: lac
replicon: pMB1

contains sequence

integrating vector

shuttle vector

vector permitting positive selection for integration

vector permitting visual detection of recombinants

Restriction digests of the clone give the following sizes (kb): EcoRI--5.2; PstI--5.2; HindIII--2.9, 2.4.

Shuttle vector for integrating sequences at leu1. Permits visual detection of recombinants by lacZalpha complementation.

Constructed from pJK142 (ATCC 86955) by inserting the leu1 gene at the NdeI site.

The leu1 gene contains the following unique restriction sites: StyI EcoNI Eco47III BsmI Bsu36I SplI Tth3I NdeI NruI SnaBI.

The order of the major feature in the plasmid is: ampR - pMB1 ori - 5' lacZ' - SacI/MCS/KpnI - 3' lacZ' - leu1.

component of:ATCC 86958

component of:ATCC 87686