| Comments |
Restriction digests of the clone give the following sizes (kb): BamHI--6.9, 0.8; EcoRI--2.7 (doublet), 2.2; KpnI--5.6, 1.45, 0.6; NcoI--3.6, 1.4, 0.9, 0.75, 0.45, 0.3, 0.25; PstI--5.2, 2.5. The insert contains the following restriction sites (approximate kb from the 5' end): BamHI--1.99; KpnI--0.15, 0.73; NcoI--0.36, 0.80, 1.66, 2.61; NotI--0.16; PstI--2.47. The EcoRI insert site in pSVL was introduced in the SmaI site. |
| References |
Van de Ven WJ, et al. Furin: the prototype mammalian subtilisin-like proprotein-processing enzyme. Endoproteolytic cleavage at paired basic residues of proproteins of the eukaryotic secretory pathway. Enzyme 45: 257-270, 1991. PubMed: 1843280
Creemers JW, et al. Life Sci. Adv. 11: 127-138, 1992.
Wim J Van De Ven, personal communication
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