Restriction digests of the clone give the following sizes (kb): EcoRI--2.7, 1.6; PvuI--4.3; PvuII--2.5, 1.8; BglI/XhoI--2.9, 1.4; ClaI/XhoI--4.3. The vector contains the following restriction sites (approximate kb from nt 1): BglI--3.07; ClaI--1.69; EcoRI--0.30, 1.71; HindIII--0.0; NcoI--0.60; PvuI--3.33; PvuII--0.20, 1.89; SacI--1.70; XhoI--0.05. Promoter-cloning vector for analysis of promoters in vertebrate cells, using the CAT reporter gene. Constructed from pBLCAT3 (ATCC 37528) by deletion of a 467 bp fragment immediately upstream from the 5' polylinker and replacement by two fragments, each containing the polyadenylation signal of the SV40 large T gene. The order of the major features in the plasmid is: two SV40 polyadenylation signals - HindIII/MCS/XhoI - cat - SV40 small T splice site - SV40 polyadenylation signal - ClaI/MCS/SstI - pMB1 ori - ampR. |
