| 產(chǎn)品名稱 | Human respiratory syncytial virus |
|---|---|
| 商品貨號 | B195016 |
| Classification | Paramyxoviridae, Pneumovirus |
| Deposited As | Respiratory syncytial virus |
| Agent | Human respiratory syncytial virus |
| Strain | 18537 |
| Applications | Respiratory research |
| Positive for Mycoplasma | No |
| Biosafety Level | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format | frozen 1 mL per vial |
| Storage Conditions | -70°C or colder |
| Images |  |
| Comments | The early passage history of this item is unknown. |
| Effect on Host | CPE, massive syncytia formation and sloughing |
| Recommended Host | HEp-2 (ATCC® CCL-23™) |
| Growth Conditions | Temperature: 37°C Recommendations for Infection: Plate cells 24-48 hours prior to infection and infect when cultures are 70-90% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.1). Adsorb 1-2 hours at 37°C in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium. Incubation: 3-7 days |
| Name of Depositor | RM Chanock, J Crowe, National Institute of Allergy and Infectious Diseases; ATCC? |
| Special Collection | ATCC |
| Source | Respiratory secretions from child with acute respiratory disease seen at Children’s Hospital of the District of Columbia, Washington, DC, 1962. Treated ATCC® VR-1401™ to remove mycoplasma at ATCC®. |
| References | Coates HV, et al. An antigenic analysis of respiratory syncytial virus isolates by a plaque reduction neutralization test. Am. J. Epidemiol. 83: 299-313, 1966. PubMed: 5933417 |
| 梅經(jīng)理 | 17280875617 | 1438578920 |
| 胡經(jīng)理 | 13345964880 | 2438244627 |
| 周經(jīng)理 | 17757487661 | 1296385441 |
| 于經(jīng)理 | 18067160830 | 2088210172 |
| 沈經(jīng)理 | 19548299266 | 2662369050 |
| 李經(jīng)理 | 13626845108 | 972239479 |
