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Mumps rubulavirus
Mumps rubulavirus
規(guī)格:
貨期:
編號(hào):B195140
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱(chēng) Mumps rubulavirus
商品貨號(hào) B195140
Classification Paramyxoviridae, Rubulavirus
Deposited As Mumps
Agent Mumps rubulavirus
Strain Jones
Common Name Mumps virus
Biosafety Level 2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Isolation
Throat washing one day after onset of parotitis
Product Format frozen
Storage Conditions -70°C or colder
Comments
VR-1438 is a mycoplasma-free strain of Mumps derived at the ATCC® from VR-365 by three passages in the presence of MP Biomedicals Mycoplasma Removal Agent, (4-oxoquinoline-3-carboxylic acid derivative). The most accurate method of titration to confirm infectivity endpoint is the hemadsorption technique. The Jones strain was passaged extensively in CE and CE TC to attenuate it for humans.
Effect on Host
Yes, in vitro effects: Cytopathic effects in tissue culture
CPE, cell degeneration, and sloughing
Recommended Host
chicken embryo fibroblasts

Recommended Host: SL-29 cells (ATCC? CRL-1590?)

chicken embryo fibroblasts; human kidney cells; marmoset kidney cells; rhesus monkey kidney cells; HEp-2 cells (ATCC CCL-23)
Growth Conditions
Temperature: 37.0°C
Duration: 5-7 days
Name of Depositor F Deinhardt, G Shramek
Special Collection NCRR Contract
Source Throat washing one day after onset of parotitis
References

Shramek G, Deinhardt F. Development of an attenuated mumps virus vaccine. II. Immune response of animals to vaccination with inactivated and live attenuated mumps viruses. J. Immunol. 102: 1093-1098, 1969. PubMed: 5768187

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