A 2.65 kb NotI - BglII fragment containing the MRP7 promoter and the GAL4-hER-VP16 construct was inserted into the NotI and BamHI sites of pRS314. The order of features in the insert are - MRP7 promoter (nt 1-1092) - GAL4 (a.a. 1-93) - Estradiol receptor-alpha (a.a. 282-576) - Herpes Simplex virus protein 16 (a.a. 424-490). The start codon begins at nt 1093. The stop codon begins at nt 2497.
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References
Tightly regulated, B-Estradiol Dose-Dependent Expression System for Yeast. BioTechniques 29: 1226-1231, 2000.
Shipped
frozen
Shipping Information
Frozen glycerol stock of Escherichia coli GC10 containing the plasmid.
