Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Isolation
Macaca nemestrina, West Malaysia
Product Format
frozen
Type Strain
yes
Comments
low-density parasitemia in Aotus lemurinus griseimbra monkeys
infection in Aotus monkeys
infection in rhesus monkeys
Observation on the relapse activity
Growth Conditions
Duration: in vivo, Macaca mulatta
Protocol: ATCCNO: 30037 SPEC: This strain is distributed as a frozen stabilate. See general instructions for thawing and storage of frozen material before proceeding. As soon as the strain arrives, remove the frozen ampule from the dry ice and transfer it directly to a 35C water bath. When it is completely thawed, aseptically remove the material with a syringe and inject the entire contents of the vial into an Aotus trivirgatus. This parasite can be maintained by serial passage of parasitized blood (by intravenous inoculation) into monkeys (Aotus trivirgatus).
Subcultivation
Protocol: ATCCNO: 30037 SPEC: This strain is distributed as a frozen stabilate. See general instructions for thawing and storage of frozen material before proceeding. As soon as the strain arrives, remove the frozen ampule from the dry ice and transfer it directly to a 35C water bath. When it is completely thawed, aseptically remove the material with a syringe and inject the entire contents of the vial into an Aotus trivirgatus. This parasite can be maintained by serial passage of parasitized blood (by intravenous inoculation) into monkeys (Aotus trivirgatus).
Name of Depositor
National Institutes of Health
References
Collins WE, Contacos PG. Observations on the relapse activity of Plasmodium fieldi in the rhesus monkey. J. Parasitol. 57: 29-32, 1971. PubMed: 4994804
Eyles DE, et al. Plasmodium fieldi sp. nov., a new species of malaria parasite from the pig-tailed macaque in Malaya. Ann. Trop. Med. Parasitol. 56: 242-247, 1962.
Collins WE, et al. Infection and transmission studies with the N-3 strain of Plasmodium fieldi in the Macaca mulatta monkey. J. Parasitol. 70: 422-427, 1984. PubMed: 6491849
Sullivan JS, et al. Attempts to transmit the N-3 strain of Plasmodium fieldi to Aotus monkeys. J. Parasitol. 84: 195-197, 1998. PubMed: 9488369
