| Insert |
DNA: genomic Insert information: Insert size (kb): ?36.146 Insert ends : MboI Genes : ??????????????? mating factor a, MFA1, ??????????????? ribosomal protein L27b, RPL27B ??????????????? negative regulator of prp genes,? SPP41 ??????????????? nuclear-localized protein containing zinc finger ?????????? motifs, involved in pre-tRNA splicing, STP1 DNA : genomic, chromosome IV R Source : Saccharomyces cerevisiae AB972 (ATCC 76269) Genbank accession : Nucleotides 1-36146 of the insert correspond to nucleotides 2618-38763 of U33050. Insert lengths(kb): 36.14599990844727 Gene product: mating factor a [MFA1] Gene product:ribosomal protein L27b (RPL27B) Gene product: negative regulator of prp genes (SPP41) Gene product: nuclear-localized protein containing zinc finger motifs, involved in pre-tRNA splicing (STP1) |
| Comments |
Reported to contain EcoRI/HindIII fragments of the following sizes (kb), ordered as in the genome: 3.077, 2.207, 2.564, 2.534, 0.728, 1.488, 2.242, 0.862, 3.562, 2.528, 2.803, 0.799, 1.821, 2.002, 1.215, 0.545, 1.168, 0.406. IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture. Overlaps clones with ATCC number(s): 70439, 70457, 70572, 70736. NOTE: Because of the large number of simultaneous deposits from Dr. Olson, the ATCC has not verified that the characteristics of the material supplied here are as published. The ATCC encourages users of this material to communicate their results to us. |
