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Distribution host: Escherichia coli S17-1

Size (kb): 3.7599999904632570

DESCRIPTION OF VECTOR:
Intact vector size: 3.760
Type of vector: plasmid
Cloning sites: HindIII SphI PstI SalI XbaI BamHI SmaI KpnI EcoRI
Polylinker sites: HindIII SphI PstI SalI XbaI BamHI SmaI KpnI EcoRI
Construction: pK19, pSUP102 (RP4 mob)
Host range: Escherichia coli; Salmonella sp.; Serratia sp.
Features (with orientation and position when available):
marker(s): kanR, ->
replicon: oriT
replicon: oriV
insert detection: lacZ', ->
MCS: HindIII...EcoRI, ->

Vector: pK19mob (plasmid)

Construction: pK19, pSUP102 (RP4 mob)

Marker(s):kanR

Construct size (kb): 3.759999990463257

Features: insert detection: lacZ'
marker(s): kanR
replicon: oriT
replicon: oriV
MCS: HindIII...EcoRI

integrating vector

mobilizable vector

vector containing primer sites useful for sequencing

vector permitting visual detection of recombinants

Restriction digests of the clone give the following sizes (kb): EcoRI--3.8; HindIII--3.8; PstI--3.8.

After mobilization, the plasmid can be maintained by integration into the host chromosome via homologous recombination.

Differs from pK18mob (ATCC 87095) only in the orientation of the polylinker.

Cloning vector allowing mobilization into a wide range of Gram- and Gram+ bacteria.

Temperature: 37.0°C