Restriction digests of the clone give the following sizes (kb): BamHI--2.7, 1.75; PvuII--2.35, 2.1; EcoRI--4.45. BglII and HindIII cut multiple times within the reading frame. A plasmid providing the gene for a yeast selectable marker in the MCS of pUC18, allowing more flexibility in designing constructions. The insert was subcloned from pSZ515. HIS3 is in the same orientation as lacZ'. |
