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hGR(1-777)

hGR(1-777)

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產(chǎn)品名稱	hGR(1-777)
商品貨號(hào)	B196156
Designations	hGR(1-777)
Species	Homo sapiens, human
Depositors	MV Govindan
Applications	in another host, produces protein glucocorticoid receptor
Vector	Construct size (kb): 8.90000057220459 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pKCR-2 Intact vector size: 5.700 Type of vector: plasmid Vector end: BamHI Vector end: BamHI Cloning sites: BamHI, EcoRI Polylinker sites: Host range: vertebrate cells; Escherichia coli Features (with orientation and position when available): marker(s): ampR promoter: SV40 replicon: pMB1 Cross references:
Insert	DNA: cDNA DESCRIPTION OF INSERT COMPONENT: Genome: Homo sapiens Gene symbol: GRL Genomic copy number: unique Gene name: glucocorticoid receptor Contains complete coding sequence?: Y Chromosome: 5; Localization: 5 q31-q32 Tissue: breast adenocarcinoma MCF7 cell line Type of DNA: cDNA Insert end: Modification: BamHI linkers Insert end: Modification: BamHI linkers Insert size (kb): 2.88 Cross references: Insert lengths(kb): 2.880000114440918 Tissue: breast adenocarcinoma MCF7 cell line Gene product: glucocorticoid receptor [GRL] Target Gene: glucocorticoid receptor
Insert Size (kb)	2.880
Media	ATCC^® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Comments	Restriction digests of the clone give the following sizes (kb): BamHI--6.2, 3.1; EcoRI--5.8, 1.8, 0.86, 0.78; KpnI--9.4; XbaI--9.4; PstI--6.8, 2.2. The orientation of the insert is: 5' BamHI, SmaI, KpnI, SacI, EcoRI, coding region for amino acids 1-777, XbaI/BamHI 3'. Cross hybridizes with a 6.8 kb rat liver mRNA. pKCR-2 is an expression vector containing SV40 early and late promoters and beta-globin splicing and polyadenylation signals. Constructed from overlapping cDNA clones.
References	LeClerc S, et al. Molecular Cloning and characterization of a factor that binds the human glucocorticoid receptor gene and represses its expression. J. Biol. Chem. 266: 17333-17340, 1991. PubMed: 1894621 Green S, Chambon P. Oestradiol induction of a glucocorticoid-responsive gene by a chimaeric receptor. Nature 325: 75-78, 1987. PubMed: 3025750 Govindan MV, et al. Cloning of the human glucocorticoid receptor cDNA. Nucleic Acids Res. 13: 8293-8304, 1985. PubMed: 2417195 Govindan MV. Specific region in hormone binding domain is essential for hormone binding and trans-activation by human androgen receptor. Mol. Endocrinol. 4: 417-427, 1990. PubMed: 2342476 Govindan MV, et al. Differential regulation of mouse mammary tumor virus-bacterial chloramphenicol acetyltransferase chimeric gene by human mineralocorticoid hormone-receptor complexes. J. Steroid Biochem. Mol. Biol. 39: 91-103, 1991. PubMed: 1648951 Govindan MV, et al. Human glucocorticoid receptor gene promoter-homologous down regulation. J. Steroid Biochem. Mol. Biol. 40: 317-323, 1991. PubMed: 1958537 Leclerc S, et al. Purification of a human glucocorticoid receptor gene promoter-binding protein. J. Biol. Chem. 266: 8711-8719, 1991. PubMed: 2026589

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