| 產品名稱 |
Parainfluenza 3 |
| 商品貨號 |
B196179 |
| Classification |
Paramyxoviridae, Paramyxovirus |
| Agent |
Parainfluenza 3 |
| Strain |
ATCC-2011-5 |
| Applications |
Respiratory research |
| Biosafety Level |
2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format |
frozen 1 mL per vial |
| Storage Conditions |
?-70°C or colder |
| Effect on Host |
CPE, refractile cell rounding in culture CPE, refractile cell rounding |
| Recommended Host |
Production Host: ?LLC-MK2 Derivative (ATCC? CCL-7.1™) LLC-MK2 Derivative (ATCC CCL-7.1) |
| Growth Conditions |
Temperature: 35°C Recommendations for Infection: Plate cells 24-48 hours in advance and infect when cultures are 80-90% confluent. Remove medium, rinse with plain EMEM, and inoculate with a small volume (e.g. 1 mL per 25 cm2) of virus diluted to provide a MOI of about 0.1. Adsorb 1-2 hours at 35°C in a humidified 5% CO2 atmosphere, rocking every 15-20 minutes to redistribute inoculum. End adsorption by adding virus growth medium. Incubation: 9-14 days in a humidified 5% CO2 atmosphere |
| Name of Depositor |
ATCC |
| Source |
Clinical sample from a patient positive for PIV 3 in Virginia, June 2011 |
