| 產品名稱 |
pMEL34 |
| 商品貨號 |
B196251 |
| Designations |
pMEL34 |
| GenBank Number |
J03581
|
| Species |
Homo sapiens, human |
| Depositors |
B Kwon |
| Vector |
Construct size (kb): 4.900000095367432 |
| Insert |
DNA: cDNA Insert lengths(kb): 1.600000023841858 Tissue: melanocyte Gene product: tyrosinase [c] Alleles: c, c, s, s, B1, B2 |
| Insert Size (kb) |
1.600 |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information |
Distributed: freeze-dried |
| Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI--3.0, 1.85; XbaI--4.9; SmaI--4.9; ClaI--4.9; HindIII--4.9. Detects genomic EcoRI fragments of 4.5, 12.0, and 14.5 kb in c(ch)/c(ch) mice. No homologous sequences were detected in albino mutants c(14Cos)/c(14Cos) or c(3H)/c(3H). Detects a mRNA of 2.4 kb (21S) in normal human melanocytes and melanotic melanoma cells. |
| References |
Spritz RA, Strunk KM. RFLP for BglII at the human tyrosinase (TYR) locus. Nucleic Acids Res. 18: 3672, 1990. PubMed: 1972982
Kwon BS, et al. Isolation and sequence of a cDNA clone for human tyrosinase that maps at the mouse c-albino locus. Proc. Natl. Acad. Sci. USA 84: 7473-7477, 1987. PubMed: 2823263
|
