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貨號：B245025
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名稱：Streptomyces scabiei (Thaxter) Lambert and Loria

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pSM143

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編號:B196322

品牌:Mingzhoubio

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產品名稱	pSM143
商品貨號	B196322
Designations	pSM143
Depositors	G Grandi
U.S. Patent	4,987,078
Disclosure	This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Biosafety Level	1
Host	Distribution host: Escherichia coli HB101
Vector Information	Size (kb): 7.3499999046325680 Vector: pSM143 (plasmid) Promoters: Promoter ErmC Construction: pUB110, pBR322 ori, fd terminator Marker(s):ampR,kanR Construct size (kb): 7.349999904632568 Features: marker(s): ampR marker(s): kanR promoter: ErmC replicon: pMB1, pUB110 restriction site: EcoRI restriction site: XbaI terminator: fd
Applications	expression vector shuttle vector
Comments	An Escherichia coli/Bacillus subtilis shuttle expression vector utilizing the Erm C promoter of pE194 and an fd terminator.
Media	ATCC^® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions	Temperature: 37.0°C
References	Grandi G, et al. Plasmid vector containing erythromycin promoter of pE 194 modified by replacement of minus 35 region to increase expression of heterologous protein. Japanese Patent 61,257,189 dated Nov 14 1986 Grandi G, et al. Plasmid vector containing erythromycin promoter of pE 194 modified by replacement of minus 35 region to increase expression of heterologous protein. European Patent 195303 dated Sep 24 1986 Grandi G, et al. Plasmid vector containing erythromycin promoter of pE 194 modified by replacement of minus 35 region to increase expression of heterologous protein. German Federal Republic Patent 3,666,838 dated Dec 14 1989 Guido Grandi, personal communication
Shipped	freeze-dried
Shipping Information	Distributed: freeze-dried

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