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produces protein calpastatin

Construct size (kb): 5.919000148773193

DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pGEX-4T-1
Intact vector size: 4.969
Type of vector: plasmid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: EcoRI SmaI SalI XhoI NotI
Polylinker sites: EcoRI SmaI SalI XhoI NotI
Other unique sites: PstI mLuI BstEII ApaI BssHII EcoRV NarI BamHI
Construction: pGEX-2T
Host range: Escherichia coli
Features (with orientation and position when available):
other: thrombin protease cleavage site
MCS: EcoRI/.../NotI
marker(s): ampR, ->
replicon: pMB1
repressor gene: lacI, ->
promoter: tac, ->
coding sequence: S. japonicum glutathione S-transferase
Cross references:

DNA: cDNA

DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: CAST
Gene name: calpastatin
Contains complete coding sequence?: N
Chromosome: 5; Localization: 5 q15-q21
Tissue: placenta
Type of DNA: cDNA
Insert end: Modification: EcoRI linker
Insert end: Modification: EcoRI linker
Insert size (kb): 0.95
Cross references: DNA Seq. Acc.: D16217
Nucleotides 1-950 of the insert correspond to
nucleotides 1435-2384 of D16217.

Insert lengths(kb): 0.949999988079071

Tissue: placenta

Gene product: calpastatin [CAST]

Target Gene: calpastatin

Restriction digests of the clone give the following sizes (kb): EcoRI--5.0, 1.0; BamHI--5.9; PstI--4.6, 1.35; HindIII--5.4, 0.5; EcoRI/PstI--4.2, 1.0, 0.7.

The insert contains the following restriction sites (approximate kb from the 5' end): HindIII--0.13, 0.54; PstI--0.66.

This clone expresses a fusion protein of GST and calpastatin (domains 3 and 4 only). This truncated product also differs from calpastatin by a substitution (G->A) at nt 1937 of D16217, resulting in a glycine to glutamate substitution (G592E).