人妻少妇偷人精品无码丨色婷婷av久久久久久久丨欧美xxxx做受性欧美88丨欧洲女人牲交视频免费丨亚洲精品久久久av无码专区

The 7.5 kb EcoRI fragment of the insert has been used to detect the TaqI RFLP at D17S122 and the dosage difference indicative of the duplication mutation associated with Charcot-Marie-Tooth disease (CMT1A).

Construct size (kb): 36.0

DESCRIPTION OF VECTOR COMPONENT:
Name of vector: SuperCos 1
Intact vector size: 7.600
Type of vector: cosmid
Vector end: BamHI
Vector end: BamHI
Cloning sites: BamHI
Polylinker sites: EcoRI NotI BamHI NotI EcoRI
Host range: vertebrate cells; Escherichia coli
Features (with orientation and position when available):
marker(s): ampR, neoR, G418R
promoter: T3, T7
replicon: pMB1, SV40
Cross references:

DNA: genomic

DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: D17S122
Genomic copy number: unique
Gene name: DNA Segment, single copy
Contains complete coding sequence?: U
Chromosome: 17; Localization: 17 p12-p11.2
Type of DNA: genomic
Insert end:
Insert end:
Insert size (kb): 29.0
Cross references:

Insert lengths(kb): 29.0

Gene product: DNA Segment, single copy [D17S122]

Alleles: A1, A1, A2, A3, B1, A2, B2

Restriction digests of the clone give the following sizes (kb): EcoRI--9.4, 7.6, 7.5, 4.4, 2.6, 1.55, 1.45 (doublet); NotI--9.4 (doublet), 7.6, 1.45; BamHI--11.5, 8.0, 6.6, 5.2, 3.5;

EcoRI/BamHI--7.6, 7.5, 5.4, 3.0, 2.7, 2.15, 1.6, 1.45 (doublet); SacII--uncut.

IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture.

The 7.5 kb EcoRI fragment of the insert has been used to detect the TaqI RFLP at D17S122 and the dosage difference indicative of the duplication mutation associated with Charcot-Marie-Tooth disease (CMT1A).