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When cotransfected into CHO cells with pSV2neo, the construct expresses the receptor.

Construct size (kb): 6.400000095367432

DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pSVL
Intact vector size: 4.889
Type of vector: plasmid
Vector end: XhoI
Vector end: XbaI
Cloning sites: XhoI XbaI SmaI XmaI SacI BamHI
Polylinker sites: XhoI XbaI SmaI XmaI SacI BamHI
Construction: pBR322, SV40
Host range: vertebrate cells; Escherichia coli
Features (with orientation and position when available):
marker(s): ampR
promoter: SV40 late
replicon: pMB1, SV40
terminator: SV40 late poly(A)
Cross references:

DNA: cDNA

DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: ADORA1
Genomic copy number: unique
Gene name: adenosine A1 receptor
Contains complete coding sequence?: Y
Chromosome: 1; Localization: 1 q32.1
Tissue: hippocampus
Type of DNA: cDNA
Insert 5' end: PstI
Insert 3' end: XbaI
Insert size (kb): 1.5
Cross references:

Insert lengths(kb): 1.5

Tissue: hippocampus

Gene product: adenosine A1 receptor [ADORA1]

Restriction digests of the clone give the following sizes (kb): PstI/XbaI--3.3, 2.3, 1.9; BamHI--6.8, 0.9; EcoRI--5.4, 2.3; HindIII--5.8, 1.35, 0.6; XhoI--7.8.

When cotransfected into CHO cells with pSV2neo, the construct expresses the receptor.

The construct includes an XhoI/PstI fragment derived from a pBluescript SK+ multiple cloning sequence. The PstI site is at the 5' end of the cDNA.

The PstI/XbaI fragment includes 350 bp of 5' untranslated and 220 bp of 3' untranslated sequence.