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Kluyveromyces lactis (Dombrowski) van der Walt

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編號:B198105

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產品名稱	Kluyveromyces lactis (Dombrowski) van der Walt
商品貨號	B198105
Strain Designations	[61, type C, CBS 2359, CCRC 21716, DSM 70799, IFO 1267, NRRL Y-1140, VKPM Y-1174]
Application	Degrades whey permeate Hydrolyzes whey lactose Produces L-methionine Produces beta-galactosidase beta-D-galactosidase Produces killer toxin Produces L-methionine from whey permeate
Biosafety Level	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Product Format	freeze-dried
Storage Conditions	Frozen: -80°C or colder Freeze-Dried: 2°C to 8°C Live Culture: See Propagation Section
Type Strain	no
Genotype	a [k1+ k2+]
Preceptrol®	no
Genome Sequenced Strain	Yes
Mating Type	Mates with ATCC^® 8651™
Ploidy	haploid
Comments	Regulation of pyruvate dehydrogenase activity by E1alpha subunit levels Resistant to killer toxin produced by Schwanniomyces occidentalis (ATCC^® 44252™) Carries two linear DNA plasmids, pGK11 and pGK12 Transcriptional regulation of beta-galactosidase gene Effect of lactose permeabilization on beta-galactosidase production Nonflocculating Killer, immune Genome sequencing strain (Genolevures Consortium, France). Sequenced mitochondrial genome.
Medium	ATCC^® Medium 200: YM agar or YM broth ATCC^® Medium 28: Emmons' modification of Sabouraud's agar ATCC^® Medium 1245: YEPD
Growth Conditions	Temperature: 24°C to 26°C Atmosphere: Typical aerobic
Sequenced Data	18S ribosomal RNA gene, partial sequence; internal transcribed spacer 1, 5.8S ribosomal RNA gene, and internal transcribed spacer 2, complete sequence; and 26S ribosomal RNA gene, partial sequence AAGGATCATTAAAGATTATGAATGAGTAGATTACTGGGGGAATCGCTGAATATGGCCTGCGCTTAATTGCGCGGCTAATTCTTGATTTTCTGCTATCAGTTTTCTTTCTCTCATCCTAAACACAATGGAGTTTTTTCTCTATGAACTACTTCCCTGGAGAGCTCGTCTCTCCAGTGGACATAAACACAAACAACATTTTGCATTATGAAAAACTATTTATCAAGAAATTTAATATTCAAAACTTTCAACAACGGATCTCTTGGTTCTCGCATCGATGAAGAACGCAGCGAATTGCGATATGTATTGTGAATTGCAGATTTTCGTGAATCATCAAATCTTTGAACGCACATTGCGCCCTCTGGTATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTCTCTCAAACCTTTGGGTTTGGTAGTGAGTGATACTCGTTTTTCGGGTTAACTTGAAAGTGGCTAGCCGTTGCCTTCTGCGTGAGCAGGGCTGCGTGTCAAGTCTATGGACTCGACTCTTGCACATCTACGTCTTAGGTTTGCGCCAATTCGTGGTAAGCTAGGGTCAATGAGCTTATAGGTGTTATAAAGACTCGCTGGTGTTTGTCTCCTTGAGGCATACGGCTTAATCAAAACTCTCAAAGTTTGACCTCAAATCAGGTAGGAATACCCGCTGAACTTAAG D1D2 region of the 26S ribosomal RNA gene CATATCAATAAGCGGAGGAAAAGAAACCAACCGGGATTGCCTTAGTAACGGCGAGTGAAGCGGCAAAAGCTCAAATTTGAAATCTGGCGTCTTCGACGTCCGAGTTGTAATTTGAAGAAGGCAACTTTGTAGCTGGTCCTTGTCTATGTTCCTTGGAACAGGACGTCATAGAGGGTGAGAATCCCGTGTGGCGAGGATCCCAGTTATTTGTAAAGTGCTTTCGACGAGTCGAGTTGTTTGGGAATGCAGCTCTAAGTGGGTGGTAAATTCCATCTAAAGCTAAATATTGGCGAGAGACCGATAGCGAACAAGTACAGTGATGGAAAGATGAAAAGAACTTTGAAAAGAGAGTGAAAAAGTACGTGAAATTGTTGAAAGGGAAGGGCATTTGATCAGACATGGCGTTTGCTTCGGCTTTCGCTGGGCCAGCATCAGTTTTAGCGGTTGGATAAATCCTCGGGAATGTGGCTCTGCTTCGGTAGAGTGTTATAGCCCGTGGGAATACAGCCAGCTGGGACTGAGGATTGCGACTTTTGTCAAGGATGCTGGCGTAATGGTTAAATGCCG
Name of Depositor	Lederle Labs.
Chain of Custody	ATCC <-- Lederle Labs. <-- N.C. Laffer 61, type C (Zygosaccharomyces lactis)
Isolation	Creamery, USA
Cross References	Nucleotide (GenBank) : KU729077 ITS including 5.8S rRNA gene Nucleotide (GenBank) : KU729173 D1/D2 region of 28S rRNA gene Nucleotide (GenBank) : X72499 nucleotide sequence of KlGGS1 gene Nucleotide (GenBank) : X81421 K.lactis gene for alpha-alpha-trehalose 1-D-glucohydrolase. Nucleotide (GenBank) : CR382121 Kluyveromyces lactis strain NRRL Y-1140 chromosome A complete sequence.
References	Gunge N, et al. Isolation and characterization of linear deoxyribonucleic acid plasmids from Kluyveromyces lactis and the plasmid-associated killer character. J. Bacteriol. 145: 382-390, 1981. PubMed: 6257636 Wickerham LJ, Burton KA. Occurrence of yeast mating types in nature1,3. J. Bacteriol. 63: 449-451, 1952. PubMed: 14938317 Lacy LR, Dickson RC. Transcriptional regulation of the Kluyveromyces lactis beta- galactosidase gene. Mol. Cell. Biol. 1: 629-634, 1981. PubMed: 9279376 . . Biotechnol. Tech. 6: 289-292, 1992. Schaffrath R, Meacock PA. Kluyveromyces lactis killer plasmid pGKL2: molecular analysis of an essential gene, ORF5. Yeast 11: 615-628, 1995. PubMed: 7483835 Bellal M, et al. Structural differences in parietal peptidomannans from flocculating strains of Kluyveromyces lactis. Can. J. Microbiol. 41: 323-335, 1995. Luyten K, et al. Disruption of the Kluyveromyces lactis GGS1 gene causes inability to grow on glucose and fructose and is suppressed by mutations that reduce sugar uptake. Eur. J. Biochem. 217: 701-713, 1993. PubMed: 8223613 Dickson RC, Markin JS. Physiological studies of beta-galactosidase induction in Kluyveromyces lactis. J. Bacteriol. 142: 777-785, 1980. PubMed: 6769910 Siso MI. Beta-galactosidase production by Kluyveromyces lactis on milk whey: batch versus fed-batch cultures. Process Biochem. 29: 565-568, 1994. Bellal M, et al. Flocculation dispersion in Kluyveromyces lactis. Process Biochem. 30: 641-648, 1995. . . Enzyme Microb. Technol. 16: 303-310, 1994. Butler AR, et al. Analysis of the response of Saccharomyces cerevisiae cells to Kluyveromyces lactis toxin. J. Gen. Microbiol. 137: 1749-1757, 1991. PubMed: 1955863 Zeeman AM, et al. Regulation of pyruvate metabolism in chemostat cultures of Kluyveromyces lactis CBS 2359. Yeast 16: 611-620, 2000. PubMed: 10806423 Chen WB, et al. Isolation, purification, and characterization of a killer protein from Schwanniomyces occidentalis. Appl. Environ. Microbiol. 66: 5348-5352, 2000. PubMed: 11097913 Kitamoto HK, Nakahara T. Isolation of an L-methionine-enriched mutant of Kluyveromyces lactis grown on whey permeate. Process Biochem. 29: 127-131, 1994. Dujon B, et al. Genome evolution in yeasts. Nature 430: 35-44, 2004. Souciet JL, et al. Genomic Exploration of the Hemiascomycetous Yeasts: 1. A set of yeast species for molecular evolution studies. FEBS Lett. 487: 3-12, 2000. Bolotin-Fukuhara M, et al. Genomic Exploration of the Hemiascomycetous Yeasts: 11. Kluyveromyces lactis. FEBS Lett. 487: 66-70, 2000. Wang H, et al. A fungal phylogeny based on 82 complete genomes using the composition vector method. BMC Evol. Biol. 9: 195, 2009. PubMed: 19664262 Zivanovic Y, et al. Complete nucleotide sequence of the mitochondrial DNA from Kluyveromyces lactis. FEMS Yeast Res. 5: 315-322, 2005. PubMed: 15691736

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