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Construct size (kb): 4.800000190734863

DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript SK-
Intact vector size: 2.960
Type of vector: phagemid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: KpnI ApaI DraII XhoI (SalI HincII AccI) ClaI HindIII EcoRV
EcoRI PstI SmaI BamHI SpeI XbaI EagI NotI SacII BstXI SacI
Polylinker sites: SacI BstXI SacII NotI EagI XbaI SpeI BamHI SmaI PstI EcoRI
EcoRV HindIII (ClaI Bsp106I) (HincII AccI SalI) XhoI
(Eco0109I DraII ApaI) KpnI
Construction: pUC19
Host range: Escherichia coli
Features (with orientation and position when available):
replicon: f1, ->
insert detection: lacZ', <-
primer site: M13-20, ->
promoter: T7, ->
MCS: KpnI...SacI, ->
promoter: T3, <-
promoter: lac, <-
primer site: M13 reverse, <-
replicon: pMB1
marker(s): ampR, <-
Cross references:

DNA: cDNA

DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: SF*
Gene name: splicing factor PR264 (SC35)
Contains complete coding sequence?: U
Chromosome: 17; Localization: 17 q25
Tissue: fetal brain, female, 17-18 wk (Stratagene 936206)
Type of DNA: cDNA
Insert end: Modification: EcoRI linkers
Insert end: Modification: EcoRI linkers
Insert size (kb): 1.8
Cross references: DNA Seq. Acc.: T07875; EST: EST05765; Primer: M13-21
DNA Seq. Acc.: X75755; EST: EST05765
Nucleotides 1-285 of the insert correspond to
nucleotides 285-1 of X75755 with 98% identity.

Insert lengths(kb): 1.799999952316284

Tissue: fetal brain, female, 17-18 wk (Stratagene 936206)

Gene product: splicing factor, arginine/serine-rich 2( myelin regulatory factor 1, splicing factor Sc35, splicing factor SC35) [SF*]

Restriction digests of the clone give the following sizes (kb): EcoRI--3.0, 1.23, 0.6.

The insert contains multiple EcoRI fragments. It is important to note these may have been derived from different cDNAs.