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LL08NS02
LL08NS02
規格:
貨期:
編號:B198807
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱 LL08NS02
商品貨號 B198807
Designations LL08NS02
Species Homo sapiens, human
Depositors M Van Dilla, JA Garnes
Origin
hamster (CHO)/human lymphocyte UV20HL21-17 hybrid
Vector
Tissue: hamster (CHO)/human lymphocyte UV20HL21-17 hybrid
Chromosome: 8
DNA: genomic
Digest: Complete
Insert size range: 4.0 kb average
Genome Equivalent: 20.0
Coverage: Clones for "complete" coverage: 24000.0
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: frozen (volume: 0.2 ml)
Comments
The peak was well-isolated from hamster background; little debris. According to fluorescence in situ hybridization using genomic hamster DNA with sorted chromosomes, there is 15% hamster DNA.
About 84% of the library is human (and 16% hamster) by plaque hybridization.
Of 52 isolates giving definitive results, 5 contained hamster sequences. One hybridized to hamster (2 fragments) and human (same size as insert) DNA.
Of 76 inserts recloned into pUC13: 8-no identifiable insert; 4-very small inserts; 38-human repeats; 3-identical to others; 2--lost; 5-hamster; 7-no hybridization; 9-human (2 multiple copy, 4/7 single copy detect RFLP, 1 on chromosome 4.
The value listed under genome equivalents is for the amplified library. Coverage was calculated by dividing the number of independent recombinants by the genome equivalents of the unamplified library. This value is 93.
References

. Human chromosome-specific DNA libraries: Construction and availability. Bio-Technology 4: 537-552, 1986.

Fuscoe JC, et al. Construction of fifteen human chromosome-specific DNA libraries from flow-purified chromosomes. Cytogenet. Cell Genet. 43: 79-86, 1986. PubMed: 3780319

Wood S, et al. Isolation and characterization of DNA probes from a flow-sorted human chromosome 8 library that detect restriction fragment length polymorphism (RFLP). Am. J. Hum. Genet. 39: 744-750, 1986. PubMed: 2879441

Perlman J, Fuscoe JC. Molecular characterization of the purity of seven human chromosome-specific DNA libraries. Cytogenet. Cell Genet. 43: 87-96, 1986. PubMed: 3780320

Deaven LL, et al. Construction of human chromosome-specific DNA libraries from flow sorted chromosomes. Cold Spring Harbor Symp. Quant. Biol. 51: 159-167, 1986. PubMed: 3472712

Fuscoe JC. Human chromosome-specific DNA libraries: use of an oligodeoxynucleotide probe to detect non-recombinants. Gene 52: 291-296, 1987. PubMed: 3609744

Marvin Van Dilla, personal communication

梅經理 17280875617 1438578920
胡經理 13345964880 2438244627
周經理 17757487661 1296385441
于經理 18067160830 2088210172
沈經理 19548299266 2662369050
李經理 13626845108 972239479
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