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Size (kb): 5.3959999084472660

Vector: pASZ10 (phagemid)

Promoters: Promoter T7

Construction: pTZ19B, ADE2#0

Marker(s):ampR,ADE2

Construct size (kb): 5.395999908447266

Features: insert detection: lacZ'
marker(s): ampR, ADE2
promoter: lac, T7
replicon: pMB1, ARS, f1

YR-type (replicating) shuttle vector

contains easily purifiable cassette(s) for construction

contains sequence phosphoribosylaminoimidazole succinocarboxamide synthetase SAICAR synthetase, phosphoribosylaminoimidazole carboxylase

expression vector

shuttle vector

vector containing primer sites useful for sequencing

vector permitting RNA synthesis in vitro

vector permitting production of single-stranded DNA

vector permitting visual detection of activity in colonies

vector permitting visual detection of recombinants

Restriction digests of the clone give the following sizes (kb): BglII--2.9, 2.6; EcoRI--5.6; HindIII--2.9, 1.3, 1.1, 0.41; BamHI--5.6.

The ADE2 segment contains HindIII sites approximately 1.17 kb and 2.36 kb from the 5' BglII site.

A YR-type shuttle expression vector containing promoters for in vitro RNA synthesis and priming sites useful for sequencing. Contains readily purifiable elements for construction experiments.

In E. coli, permits visual detection of recombinants and production of ssDNA. Encodes the lacZalpha (lacZ') peptide.

The naturally occurring BglII site within ADE2 was destroyed by replacing the A at nt 1243 of the reported sequence with a G. A BglII linker was added to the 5' end,

and the naturally occurring BglII site at the 3' end (nt 2512) was used to create a BglII fragment containing the complete coding sequence.

pTZ19B was derived from pTZ19R by adding a BglII linker at coordinate 416.

Constructed by inserting a 2.5 kb fragment (Ars+ Ade2+) into the BglII site of pTZ19B.

The order of the major features in this plasmid is: lacZ'/MCS - T7 promoter - ADE2 - pMB1 ori - ampR - f1 ori.

Temperature: 37.0°C