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Distribution host: Escherichia coli unknown (ATCC 33694)

Size (kb): 6.352

DESCRIPTION OF VECTOR:
Intact vector size: 6.352
Type of vector: phagemid
Cloning sites:
Polylinker sites:
Other unique sites: BamHI
Construction: pRS406
Host range: Saccharomyces cerevisiae; Escherichia coli
Features (with orientation and position when available):
marker(s): URA3, ->
replicon: f1, <-
other: LYS2 deleter cassette
replicon: pMB1
marker(s): ampR, <-

Vector: pAD2 (phagemid)

Construction: pRS406

Marker(s):URA3,ampR

Construct size (kb): 6.352

Features: marker(s): URA3
marker(s): ampR
other: LYS2 deleter cassette
replicon: f1
replicon: pMB1

YI-type (integrating) shuttle vector

marker deletion vector 2-aminoadipate reductase

Restriction digests of the clone give the following sizes (kb): KpnI--6.3; KpnI/SstI--4.3, 2.0; ClaI--6.3.

The two step selection process requires a ura3 transformation host (this host can be created using pJL164 (ATCC 87471)). After transformation with the ClaI linearized vector, URA3 integrants are selected on ura- plates.

The designer deletion strain is then recovered by selection on 5-FOA plates (loss of URA3 and LYS2 markers by a homologous recombination event).

Deleter vector for constructing designer yeast strains with a non-revertable deletion of a lys2 auxotrophic marker.

Temperature: 37°C

component of:ATCC 87472