aortic endothelial and adipose mesenchymal stem cell
Product Format
frozen Ten 1 mL vials of cells Five 200 mL liquid medium kit
Morphology
endothelial- and mesenchymal-like
Culture Properties
adherent
Biosafety Level
2 [Cells containing SV40 viral DNA sequences]
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease
normal
Age
23 (TeloHAEC-GFP); N/A for hTERT-MSC
Gender
both female
Ethnicity
both Caucasian
Applications
In vitro angiogenesis assay for drug screening, toxicity, pathway research, and gene function study
Shipping Information
10 frozen 1 mL vials of cells (Angio-Ready? Cells ACS-2007?) and five 200 mL liquid medium kit, Angio-Ready? Angiogenesis Medium with VEGF Supplement (ACS-2008), shipped on dry ice
Storage Conditions
cells at -130oC; medium at -20oC
Karyotype
normal
Derivation
Derived from aortic and adipose tissue; a proprietary mix of TeloHAEC-GFP (ATCC CRL-4054) and ASC52telo (ATCC SCRC-4000) hTERT-immortalized cells
Antigen Expression
CD31 and CD90
Oncogene
may contain viral sequences
Comments
For details regarding Angio-Ready Angiogenesis Medium with VEGF Supplement see ATCC® ACS-2008
Complete Growth Medium
Angio-Ready™ Angiogenesis Medium with VEGF Supplement (ATCC® ACS-2008)
Seeding Density
7.5 x 104 per well of a 96-well plate (for other size plates refer to protocol)
Cryopreservation
90% FBS + 10% DMSO
Cells per Vial
7.5X106
Volume
Ten 1 mL vials of cells Five 200 mL liquid medium kit
COI
Human
Sterility Tests
none detected
Viral Testing
none detected
Functional Tests
Pass – tubular formation assay
Population Doubling Time
N/A
Name of Depositor
ACS
Year of Origin
N/A
Year of Deposit
N/A
References
Hsu CW, et al. Identification of approved and investigational drugs that inhibit hypoxia-inducible factor-1 signaling. Oncotarget 7(7): 8172-8183, 2016. PubMed: 26882567
