人妻少妇偷人精品无码丨色婷婷av久久久久久久丨欧美xxxx做受性欧美88丨欧洲女人牲交视频免费丨亚洲精品久久久av无码专区

熱門搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
購物車 1 種商品 - 共0元
當前位置: 首頁 > ATCC代理 > CCD-1141Sk
最近瀏覽歷史
聯系我們
  • 0574-87157013
  • mingzhoubio@163.com
  • 浙江省寧波市鎮海區莊市街道興莊路9號
  • 創e慧谷42號樓B幢401室
CCD-1141Sk
CCD-1141Sk
規格:
貨期:
編號:B199533
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱 CCD-1141Sk
商品貨號 B199533
Organism Homo sapiens, human
Tissue skin
Cell Type fibroblast
Product Format frozen
Morphology fibroblast
Culture Properties adherent
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease normal
Age 44 years
Gender male
Ethnicity Caucasian
Applications
The line was established from normal skin taken from the abdomen.
Storage Conditions liquid nitrogen vapor phase
Images
Derivation
The line was established from normal skin taken from the abdomen.
Clinical Data
male
Caucasian
44 years
Comments
The line was established from normal skin taken from the abdomen.
Cells senesce after approximately 50 population doublings.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Iscove's Modified Dulbecco's Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Protocol:
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
  3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: Every 2 to 3 days
Cryopreservation
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Name of Depositor F.Haq, ATCC
Year of Origin August, 2003
梅經理 17280875617 1438578920
胡經理 13345964880 2438244627
周經理 17757487661 1296385441
于經理 18067160830 2088210172
沈經理 19548299266 2662369050
李經理 13626845108 972239479
主站蜘蛛池模板: 国产女人喷潮视频免费| 欧美熟妇性xxx交潮喷| 性史性农村dvd毛片| 国产欧美另类久久精品蜜芽 | 看成年全黄大色黄大片| 女人被狂躁高潮啊的视频在线看| 亚洲国产精品va在线看黑人 | 国产性猛交普通话对白| 99精品丰满人妻无码a片| 亚洲成 人 综合 亚洲欧洲| 男女后式激烈动态图片| 麻花传媒mv在线观看| 岛国av无码免费无禁网站麦芽| 男女啪啪高清无遮挡免费| 性高湖久久久久久久久aaaaa| 欧美激情一区二区三区在线| 国产精品无码av无码| 久久66热人妻偷产精品| 国产成人无码午夜视频在线播放| 18禁黄无遮挡网站| 69天堂人成无码免费视频| 午夜嘿嘿嘿在线观看| 国产乱xxⅹxx国语对白| 性乌克兰xxxx极品| 久久 国产 尿 小便 嘘嘘| 最近最好的中文字幕2019免费| 国产真人性做爰久久网站| 又粗又黄又硬又爽的免费视频| 一区三区不卡高清影视| 97婷婷狠狠成为人免费视频| 亚洲国产精品va在线看黑人| 亚洲欧美日韩成人一区| av蓝导航精品导航| 亚洲hdmi高清线| 天堂在线观看www| 丁香婷婷综合久久来来去| 亚洲精品少妇一区二区 | 欧美肥婆性猛交xxxx| 小草社区视频在线观看| 国产成人久久精品一区二区三区| 中文字幕乱码一区av久久不卡|