pWR3.26 [molecular clone of rhinovirus 14] purified plasmid DNA
商品貨號(hào)
B200086
Designations
pWR3.26 [molecular clone of rhinovirus 14] purified plasmid DNA
GenBank Definition
Human rhinovirus type 14 polyprotein gene.
GenBank Number
L05355
Species
Human rhinovirus 14, Human rhinovirus 14
Depositors
W Lee
Applications
contains sequence
Vector
Type of vector: E. coliName of vector: pUC-19
Intact vector size (kb): unknown at this time
Markers (ie. AMPR): AMPRCloning site: The HRV14 cDNA was transplanted into the vector at the StuI site, then additional modifications were made to the viral sequence by site-directed mutagenesis. (See reference article)
HindIII - 3 sites; Fragment lengths: 5338, 2362, 2147
MluI- 1 site; Fragment length: 9847
Biosafety Level
2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Comments
The construct contains a full-length Human Rhinovirus type 14 (HRV14) genome cloned into the StuI site of the pMJ3 vector.
HRV14 cDNA was transplanted into the vector at the StuI site.
The 5' end of the cDNA is two bases (GG) from the transcription initiation site of the T7 promoter. A unique MluI site (ACGCGT) was added after the polyA tail. Full length transcripts can be produced using T7 RNA polymerase. T7 transcripts are half as infective as genomic RNA from virons.
Classification
Picornaviridae, Enterovirus, Rhinovirus B
References
Lee WM, et al. Role of maturation cleavage in infectivity of picornaviruses: activation of an infectosome. J. Virol. 67: 2110-2122, 1993. PubMed: 8383233
