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Size (kb): 14.6000003814697300

Vector: pdBPV-MMTneo(342-12) (plasmid)

Promoters: Promoter MMT (mouse metallothionein I)

Construction: pML2, BPV-1, Tn5, mouse

Marker(s):G418R,ampR

Construct size (kb): 14.60000038146973

Features: marker(s): G418R
marker(s): ampR
other: SV40 splicing and 3' processing signals
promoter: MMT (mouse metallothionein I)
replicon: complete BPV-1 genome
replicon: pMB1
restriction site: BamHI
restriction site: BglII
restriction site: EcoRI

shuttle vector

vector permitting positive selection for integration

Restriction digests of the clone give the following sizes (kb): BglII--7.8, 4.0, 2.4, 0.7; EcoRI--9.4, 4.8; HindIII--8.6, 6.4.

A stable extrachromosomal plasmid which can be positively selected for in mouse cells.

Temperature: 37.0°C