| 產品名稱 | SW10 |
|---|---|
| 商品貨號 | B201215 |
| Organism | Mus musculus, mouse |
| Cell Type | neuronal Schwann cell; immortalized with SV40 large T antigen |
| Product Format | frozen |
| Morphology | neuronal |
| Culture Properties | adherent |
| Biosafety Level | 2 [Cells contain SV40 viral DNA sequences]
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Storage Conditions | liquid nitrogen vapor phase |
| Images |  |
| Derivation | Primary Schwann cells were infected with a retroviral vector carrying a temperature sensitive SV40 large T antigen. |
| Genes Expressed | Myelin-associated glycoprotein (Mag); protein, negative Ref Myelin basic protein (Mbp); protein, negative Ref Myelin protein zero (Mpz) (Charcot-Marie-Tooth neuropathy 1B); protein, negative Ref Peripheral myelin protein 22 (Pmp22); protein, negative Ref S100 calcium-binding protein, beta (neural) (S100b); protein, positive Ref |
| Comments | The cells can be cultured at the permissive temperature of 33°C (proliferative) or the restrictive temperature of 37°C (nonproliferative). Ref A culture submitted to the ATCC in December of 2002 was found to be contaminated with mycoplasma. Progeny were cured by a 21-day treatment with BM Cycline. The cells were assayed for mycoplasma, by the Hoechst stain, PCR and the standard culture test, after a six-week period following treatment. All tests were negative. Primary Schwann cells were infected with a retroviral vector carrying a temperature sensitive SV40 large T antigen.
|
| Complete Growth Medium | The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subcultivation Ratio: 1:4 to 1:8 Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a Manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994. |
| Cryopreservation | Complete growth medium, 95%; DMSO, 5%. Cell culture tested DMSO is available as ATCC Catalog No. 4-X. |
| Culture Conditions | Temperature: 33°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5% |
| Temperature Effects | Restrictive temperature: 37°C yes
Permissive temperature: 33°C yes
|
| Name of Depositor | PI Patel |
| References | Hai M, et al. Comparative analysis of Schwann cell lines as model systems for myelin gene transcription studies. J. Neurosci. Res. 69: 497-508, 2002. PubMed: 12210843 |
| 梅經理 | 17280875617 | 1438578920 |
| 胡經理 | 13345964880 | 2438244627 |
| 周經理 | 17757487661 | 1296385441 |
| 于經理 | 18067160830 | 2088210172 |
| 沈經理 | 19548299266 | 2662369050 |
| 李經理 | 13626845108 | 972239479 |
Hai M, et al. Comparative analysis of Schwann cell lines as model systems for myelin gene transcription studies. J. Neurosci. Res. 69: 497-508, 2002. PubMed: 12210843