| Comments |
Animals were immunized with TC-31 (ATCC CRL-2494), a monoclonal antibody to exotoxin A of Pseudomonas aeruginosa. Spleen cells were fused with Sp2/0-Ag14 myeloma cells. The antibody binds specifically to an idiotypic determinant on TC-31 anti-exotoxin A monoclonal antibody. Exotoxin A inhibits the binding of the 9-2-4 antibody to TC-31. This antibody conjugated to keyhole limpet hemocyanin (KLH) can induce antibodies in mice that bind to exotoxin A and protect 80% of the mice from a lethal injection of exotoxin A. |
| References |
Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.
Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.
Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.
Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online.
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