ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Comments
Restriction digests of the clone give the following sizes (kb): SacI--3.9; SalI--2.8, 1.1; XbaI--2.3, 1.4.
The insert contains the following restriction sites (approximate kb from the 5' end): BglI--0.18, 0.37; SalI--0.34; SacI--1.11.
Digestion with XbaI yields an intact insert with a portion of the T7 phi 10 promoter included at the 5' end and a portion of the MCS at the 3' end. Extraneous 3' material can be removed with an EcoRI digest.
The pH 2.5 acid phosphatase is more active as a phytase.
Classification
Enterobacteriaceae, Escherichia
References
Ostanin K, et al. Overexpression, site-directed mutagenesis, and mechanism of Escherichia coli acid phosphatase. J. Biol. Chem. 267: 22830-22836, 1992. PubMed: 1429631
