| 產品名稱 |
6-6-1 |
| 商品貨號 |
B203610 |
| Designations |
6-6-1 |
| GenBank Number |
M90104
|
| Species |
Homo sapiens, human |
| Depositors |
T Maniatis |
| Vector |
Construct size (kb): 3.299999952316284 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pSP73
Intact vector size: 2.464
Type of vector: plasmid
Vector end: EcoRV
Vector end: HindIII
Cloning sites: BglII EcoRV ClaI EcoRI SacI KpnI SmaI BamHI XbaI SalI AccI
PstI SphI HindIII PvuII XhoI
Polylinker sites: BglII EcoRV ClaI EcoRI SacI KpnI SmaI BamHI XbaI SalI AccI
PstI SphI HindIII PvuII XhoI
Host range: Escherichia coli
Features (with orientation and position when available):
promoter for in vitro transcription: SP6, ->
MCS: XhoI...BglII, ->
promoter for in vitro transcription: T7, <-
replicon: pMB1
marker(s): ampR
Cross references: DNA Seq. Acc.: X65333 |
| Insert |
DNA: cDNA DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: SFRS2
Other gene symbols: SC35
Gene name: splicing factor, arginine/serine-rich 2
Contains complete coding sequence?: Y
Tissue: B lymphocyte
Type of DNA: cDNA
Insert 5' end:
Insert 3' end: HindIII
Insert size (kb): 0.83
Cross references: DNA Seq. Acc.: M90104
Nucleotides 1-823 of the insert correspond to
nucleotides 142-964 of M90104. Insert lengths(kb): 0.8299999833106995 Tissue: B lymphocyte Gene product: splicing factor, arginine/serine-rich 2( myelin regulatory factor 1, splicing factor Sc35, splicing factor SC35) [SC35] |
| Insert Size (kb) |
0.830 |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information |
Distributed: freeze-dried |
| Comments |
Restriction digests of the clone give the following sizes (kb): EcoRV/HindIII--2.4, 0.83; EcoRV--3.3; HindIII--3.3; BglI--3.3; BglII--2.7, 0.62. |
| References |
Fu XD, Maniatis T. Isolation of a complementary DNA that encodes the mammalian splicing factor SC35. Science 256: 535-538, 1992. PubMed: 1373910
|
