| 產品名稱 |
PKCzeta(FC23) |
| 商品貨號 |
B204084 |
| Designations |
PKCzeta(FC23) |
| GenBank Number |
L14283
|
| Species |
Homo sapiens, human |
| Depositors |
DJ Burns |
| Vector |
Construct size (kb): 5.0 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript SK-
Intact vector size: 2.960
Type of vector: phagemid
Vector end: SpeI
Vector end: EcoRI
Cloning sites: KpnI ApaI DraII XhoI (SalI HincII AccI) ClaI HindIII EcoRV
EcoRI PstI SmaI BamHI SpeI XbaI EagI NotI SacII BstXI SacI
Polylinker sites: SacI BstXI SacII NotI EagI XbaI SpeI BamHI SmaI PstI EcoRI
EcoRV HindIII (ClaI Bsp106I) (HincII AccI SalI) XhoI
(Eco0109I DraII ApaI) KpnI
Construction: pUC19
Host range: Escherichia coli
Features (with orientation and position when available):
replicon: f1, ->
insert detection: lacZ', <-
primer site: M13-20, ->
promoter: T7, ->
MCS: KpnI...SacI, ->
promoter: T3, <-
promoter: lac, <-
primer site: M13 reverse, <-
replicon: pMB1
marker(s): ampR, <-
Cross references: |
| Insert |
DNA: cDNA DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: PRKCZ
Gene name: protein kinase C, zeta
Contains complete coding sequence?: Y
Tissue: brain, frontal cortex
Type of DNA: cDNA
Insert 5' end: SpeI
Insert 3' end: EcoRI
Insert size (kb): 2.0
Cross references: DNA Seq. Acc.: L14283 Insert lengths(kb): 2.0 Tissue: brain, frontal cortex Gene product: protein kinase C, zeta [PRKCZ] |
| Insert Size (kb) |
2.0 |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information |
Distributed: freeze-dried |
| Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI/XbaI--3.0, 2.1; BglII--5.0; PstI--4.8, 0.25; PvuII--2.5, 1.9, 0.7; SacI--4.9, 0.2. Contains the complete coding sequence only (nt 1-1779). |
| References |
Barbee JL, et al. The cDNA sequence encoding human protein kinase C-zeta. Gene 132: 305-306, 1993. PubMed: 8224878
David J Burns, personal communication
|
