Restriction digests of the clone give the following sizes (kb): AvaI--6.5, 2.8; SalI--9.3; PstI--6.4, 1.2 (doublet); HindIII--5.8, 3.1; EcoRI--5.6, 3.0. IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture. This is one of 4 probes from cosmid 1-11 which reveals RFLPs at D12S6. Using all 4 probes, the PIC of the locus is 0.73. This polymorphism is also detected by BamHI and HindIII. Constructed by inserting Sau3A fragment of cosmid 1-11 into BamHI site. |
