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pOR3
pOR3
規格:
貨期:
編號:B204189
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱 pOR3
商品貨號 B204189
Designations pOR3
GenBank Number

M11457

Species Homo sapiens, human
Depositors P Chambon
Vector
Construct size (kb): 5.599999904632568
Insert
DNA: cDNA
Insert lengths(kb): 1.299999952316284
Tissue: breast adenocarcinoma MCF7 cell line
Gene product: estrogen receptor [ESR]
Alleles: s, b, A1, A2
Insert Size (kb) 1.300
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--4.4, 1.35; BamHI--5.8; PstI--3.8, 1.04, 0.98; HindIII--5.3, 0.4; AvaI--3.0, 2.6.
Extends approximately from nt 300 - 1600 of the published sequence.
Contains sequences encoding the epitope recognized by MAb H226, believed to recognize the DNA-binding domain. This DNA-binding domain is in a 900 bp EcoRI/HindIII fragment and a 380 bp BalI fragment.
Includes sequences homologous to v-erb-A (aa 185-251).
References

Justice MJ, et al. A genetic linkage map of mouse chromosome 10: localization of eighteen molecular markers using a single interspecific backcross. Genetics 125: 855-866, 1990. PubMed: 1975791

Castagnoli A, et al. PvuII RFLP inside the human estrogen receptor gene. Nucleic Acids Res. 15: 866, 1987. PubMed: 2881267

Walter P, et al. Cloning of the human estrogen receptor cDNA. Proc. Natl. Acad. Sci. USA 82: 7889-7893, 1985. PubMed: 3865204

Green S, et al. Human oestrogen receptor cDNA: sequence, expression and homology to v-erb-A. Nature 320: 134-139, 1986. PubMed: 3754034

Kumar V, et al. Functional domains of the human estrogen receptor. Cell 51: 941-951, 1987. PubMed: 3690665

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