Restriction digests of the clone give the following sizes (kb): EcoRI--2.7, 1.21; HindIII--2.8, 1.02; PstI--2.85, 0.95; SmaI--3.9; BamHI--3.9. Contains about 36 bp 5' untranslated sequence, MBP coding region (516 bp) and 661 bp of the 1603 bp 3' untranslated region. Recorded as not polymorphic by Collaborative Research, meaning either not polymorphic in 0.8% agarose gels, or difficult to work with. PvuII RFLP has demonstrated co-dominant inheritance in 1 informative family. BamHI RFLP has demonstrated co-dominant inheritance in 1 informative family. |
