| 產品名稱 |
pZZ2 |
| 商品貨號 |
B204835 |
| Designations |
pZZ2 |
| Species |
Escherichia coli (Migula) Castellani and Chalmers |
| Depositors |
SJ Elledge |
| Applications |
mutation detection beta-galactosidase beta-D-galactosidase |
| Vector |
Construct size (kb): 11.5 |
| Insert |
DNA: genomic Insert lengths(kb): 0.8999999761581421 Gene product: ribonucleotide reductase (ribonucleoside-diphosphate reductase) large subunit(ribonucleotide reductase, large subunit) [RNR3] Target Gene: beta-galactosidase |
| Insert Size (kb) |
0.900 |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments |
Restriction digests of the clone give the following sizes (kb): BamHI/XhoI--11.0, 0.9; EcoRI--12.0; SalI--12.0. An RNR3-lacZ transcriptional fusion construct wherein beta-galactosidase expression is regulated by the RNR3 promoter. Includes up to codon 61 of RNR3. Can be used to assay RNR3 activity, such as that induced by DNA damage. |
| Classification |
Enterobacteriaceae, Escherichia |
| References |
Zhou Z, Elledge SJ. Isolation of crt mutants constitutive for transcription of the DNA damage inducible gene RNR3 in Saccharomyces cerevisiae. Genetics 131: 851-866, 1992. PubMed: 1516817
|
