Restriction digests of the clone give the following sizes (kb): BamHI--5.8; EcoRI--5.8. IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture. One of a family of cosmid vectors (ATCC 37568-37571) containing a mutated R6K origin for increased copy number. Has increased copy number over pcos5EMBL (ATCC 37570). |
