| 產(chǎn)品名稱(chēng) |
pCF1/C81 |
| 商品貨號(hào) |
B205148 |
| Designations |
pCF1/C81 |
| Species |
Hepatitis C virus |
| Depositors |
Chiron Corp., L Chung, Chiron Corp. |
| Applications |
expresses antigenic determinant nonstructural protein 4 |
| Vector |
Construct size (kb): 3.549999952316284 |
| Insert |
DNA: cDNA Insert lengths(kb): 0.3529999852180481 Gene product: nonstructural protein 4 [NS4] Target Gene: nonstructural protein 4 |
| Insert Size (kb) |
0.353 |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments |
Expression of the SOD/HCV fusion protein is inducible by IPTG. The hepatitis C virus (HCV) insert encodes amino acids 1689-1805 of the HCV polyprotein. The pSODCF1 vector was modified by removing the small BamHI/EcoRI fragment (thus losing the internal SmaI site), and then inserting a linker that restored the BamHI and EcoRI sites in the appropriate reading frame, and introduced a downstream AflII site. |
| References |
Houghton M, et al. HCV immunoassays employing C domain antigens. US Patent 5,350,671 dated Sep 27 1994
Choo QL, et al. Isolation of a cDNA clone derived form a blood-bourne Non-A, Non-B viral hepatitis genome. Science 244: 359-362, 1989. PubMed: 2523562
|
| Disclosure |
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC. |
