DESCRIPTION OF VECTOR COMPONENT: Name of vector: pBR322 Intact vector size: 4.363 Type of vector: plasmid Cloning sites: EcoRI ClaI HindIII EcoRV BamHI SphI SalI XmaIII NruI BspMI BsmI StyI AvaI BalI BspMII PvuII Tth111I NdeI AflIII PpaI PstI PvuI ScaI SspI AatII Polylinker sites: Construction: pBR313 Host range: Escherichia coli Features (with orientation and position when available): marker(s): tetR replicon: pMB1 marker(s): ampR Cross references: DNA Seq. Acc.: J01749
Insert
DNA: genomic
DESCRIPTION OF INSERT COMPONENT: Genome: feline leukemia virus (FeLV) Gene symbol: env Gene name: env Contains complete coding sequence?: U Type of DNA: genomic Insert end: Insert end: Insert size (kb): Cross references:
Gene product: env [env]
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Shipping Information
Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--3.7, 1.3; PstI--4.4.
Encodes approximately 240 amino acids from the C-terminus of FeLV gp70.
References
Nunberg JH. Feline leukemia virus vaccine plamids for fusion protein of the gp70 envelope protein of FELV. US Patent 4,701,416 dated Oct 20 1987
Disclosure
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC.
