| Vector |
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript SK-
Intact vector size: 2.960
Type of vector: phagemid
Vector end: EcoRI
Vector end: XhoI
Cloning sites: KpnI ApaI DraII XhoI (SalI HincII AccI) ClaI HindIII EcoRV
EcoRI PstI SmaI BamHI SpeI XbaI EagI NotI SacII BstXI SacI
Polylinker sites: SacI BstXI SacII NotI EagI XbaI SpeI BamHI SmaI PstI EcoRI
EcoRV HindIII (ClaI Bsp106I) (HincII AccI SalI) XhoI
(Eco0109I DraII ApaI) KpnI
Construction: pUC19
Host range: Escherichia coli
Features (with orientation and position when available):
replicon: f1, ->
insert detection: lacZ', <-
primer site: M13-20, ->
promoter: T7, ->
MCS: KpnI...SacI, ->
promoter: T3, <-
promoter: lac, <-
primer site: M13 reverse, <-
replicon: pMB1
marker(s): ampR, <-
Cross references: |
| Insert |
DNA: cDNA DESCRIPTION OF INSERT COMPONENT:
Genome: rat
Gene name: DNA Segment, expressed
Contains complete coding sequence?: U
Strain: Sprague-Dawley
Tissue: incisor, maxillary and mandibular (apical portion and
entire pulp) from animals 3-4 weeks old
Type of DNA: cDNA
Insert 5' end: EcoRI Modification: EcoRI adaptor
Insert 3' end: XhoI Modification: XhoI primer
Insert size (kb):
Cross references: DNA Seq. Acc.: R47014 Tissue: incisor, maxillary and mandibular (apical portion and entire pulp) from animals 3-4 weeks old Gene product: pyruvate kinase |
